食品与发酵工业2015，Vol.41Issue(1)：34-39,6.DOI:10.13995/j.cnki.11-1802/ts.201501007
α-胡萝卜素降解产香菌株的分离、鉴定及发酵条件优化
Isolation and identification of a α-carotenes-degradating strain and optimization of its fermentation conditions
摘要
Abstract
A high efficient α-carotenes-degradating strain ULI3 was isolated from tobacco leaf with spread plate and streak plate methods.The strain was cultured in the fementation medium containing β-carotene (15 mg/L).The degradation rate of substrates reached 89.74％ and the main degradation product included 5,6-epoxy-β-ionone (6.15％),β-ionone (5.1％),dihydroactinidiolide (3.12％) and other flavouring substances.This strain ULI3 was identified preliminarily as Saccharomyces cerevisiae through the analysis of classical morphology and ITS sequence of phylogenetic tree.On the basis of single factor test with orthogonal experiment,the optimum culture conditions of β-carotenes degradation of strain ULI3 were obtained as follows:sucrose concentration of 30 g/L,sodium nitrate concentration of 4 g/L,yeast powder concentration of 3 g/L and initial pH of 8.Under these conditions,the degradation rate of β-carotenes could reach 97.13％.关键词
α-胡萝卜素/香料物质/分离鉴定/降解率Key words
α-carotene / flavouring substances / isolation and identification / degradation rate引用本文复制引用
贾蓓蕾,魏涛,黄申,贾春晓,杨靖,张改红,白冰,毛多斌..α-胡萝卜素降解产香菌株的分离、鉴定及发酵条件优化[J].食品与发酵工业,2015,41(1):34-39,6.基金项目
国家自然科学基金(21176229)资助项目 （21176229）
