食品与发酵工业2015，Vol.41Issue(1)：142-146,5.DOI:10.13995/j.cnki.11-1802/ts.201501027
鸡软骨中硫酸软骨素的分离纯化
Optimization the purification conditions of chondroitin sulfate in chicken cartilage
摘要
Abstract
The anion exchange resin and gel chromatography method was used to purification chondroitin sulfate.The anion exchange resin D218 was selected to purify chondroitin sulfate by the way of static adsorption and analysis experiments.The best purification conditions of chondroitin sulfate was determined through dynamic adsorption and analysis was as follows:The rate of adsorption amount of liquid and resin volume was 1 ∶ 1,the adsorption velocity was 1.0 mL/min,the adsorption time was 60 min,the eluant of NaCl solution was 3.0 mol/L,the amount of eluant and resin volume ratio of 5 ∶ 1,the analysis time was 75 min,the purity of chondroitin sulfate was 72.03 ％.SephadexG75 was selected to purify chondroitin sulfate again,,the purity of chondroitin sulfate was 99.30％ under this condition,compared with the first purification improved 27.27％.The result showed that the substance was CS that was extracted from chicken cartilage through the method of HPLC.关键词
鸡软骨/硫酸软骨素/离子交换树脂/凝胶Key words
chicken cartilage/ chondroitin sulfate/ ion exchange resin/ sephadex引用本文复制引用
王鑫,徐丽萍,宋志鹏..鸡软骨中硫酸软骨素的分离纯化[J].食品与发酵工业,2015,41(1):142-146,5.基金项目
黑龙江省教育厅项目(No.12541194) （No.12541194）
黑龙江省高等教育青年专项课题(No.14Q067) （No.14Q067）
