食品与发酵工业2015，Vol.41Issue(1)：234-238,5.DOI:10.13995/j.cnki.11-1802/ts.201501045
马黛茶多酚加压毛细管电色谱法的定量分析
Simultaneous quantification of phenolic compounds in Mate tea (llex paraguariensis) by pCEC
摘要
Abstract
To establish an analytical method of simultaneous determining of neochlorogenic acid,chlorogenicacid,isochlorogenic acid A,isochlorogenic acid C-four bioactive compounds from Mate Tea(llex paraguariensis) usingpCEC (pressurized capillary electrochromatogaphy).The mobile phase was composed of methanol and acetonitrilecontaining phosphoricacid (pH 2.5,2 mmol/L) with the flow rate of 0.07 mL/min.Chromatography was monitored at wavelength 325 nm and the separation voltage was-10 kV.The contents of four phenolic components in 3 batches of samples collected from different typeswere determined and compared.The correlation coefficients between concentrations and chromatographic peak areas of neochlorogenic acid,chlorogenic acid,isochlorogenic acid A,isochlorogenic acid C wereover 0.999 and the linage ranges were 0.072 ～ 1.2,0.048 ～ 0.8,0.072 ～ 1.2,0.17 ～ 2.8mg/mL,respectively.The precisionwas 1.10％,2.12％,2.98％ and 2.32％,respectively.The repeatabilitywas 1.10％,2.12％,2.98％,2.20％,respectively.The average recoveries of the four compounds were 98.86％,99.03％,99.48％ and 99.21％,respectively.Themethod is sensitive,accurate,and can be used for the analysis of phenolic compounds from Mate tea(Ilex paraguariensis).Compared with HPLC,organic reagents used in the experiment,the dosage were much lower than normal dosage of HPLC detection.The method has great feasibility in the quality control of polyphenols and their mass composition detection.关键词
马黛茶/多酚/加压毛细管电色谱法/定量分析Key words
Mate tea (Ilex paraguarienses) / phenoliccompounds / pressurized capillary electrochromatogaphy / quantification引用本文复制引用
赵琪,臧晓韵,胡海峰..马黛茶多酚加压毛细管电色谱法的定量分析[J].食品与发酵工业,2015,41(1):234-238,5.基金项目
国家重大科学仪器设备开发专项 （）
(2011YQ15007208) （2011YQ15007208）
国家重大专项(2014zx09201001-012) （2014zx09201001-012）
“十二五”大平台子课题(X-2-3)和上海市科技支撑项目(12431901102) （X-2-3）
国家重大新药创制(2012ZX09301002-003) （2012ZX09301002-003）
