食品与发酵工业2015，Vol.41Issue(2)：1-6,6.DOI:10.13995/j.cnki.11-1802/ts.201502001
匍枝根霉β-葡萄糖苷酶BGLⅢ关键位点的结构功能
Structure and function of the key site of β-glucosidase BGLⅢ from Rhizopus stolonifer
摘要
Abstract
A novel β-glucosidase gene was cloned from genomic DNA of the filamentous fungus Rhizopus stolonifer var.reflexus TP-02 and expressed in Escherichia coli BL21.Structure and function of the gene product β-glucosidase (abbreviated as BGLⅢ) were analyzed by a series of bioinformatics software.Trp127 was considered to be the key amino acid in the enzymatic process.Results from structure analysis indicated that the conformation of Trp127 had a significant impact on the structure and function of the active center of BGLⅢ.Leu could maintain the original conformation of active centre,meanwhile,increase hydrophobic interaction with glucose,thus it could increase the efficiency of the enzyme and substrate binding.The fermentation characteristics of recombinants showed that W127A without side chain greatly decreased the activity,and W127F with benzene ring side chain did not change the activity greatly,while W127L with long chain improved the activity greatly.关键词
匍枝根霉/β-葡萄糖苷酶/结构与功能/突变Key words
Rhizopus stolonifer/ β-glucosidase / functional analysis / mutation引用本文复制引用
李娟,汤斌,李松,孟庆婷..匍枝根霉β-葡萄糖苷酶BGLⅢ关键位点的结构功能[J].食品与发酵工业,2015,41(2):1-6,6.基金项目
国家自然科学基金项目(31270135) （31270135）
