食品与发酵工业2015，Vol.41Issue(2)：89-94,6.DOI:10.13995/j.cnki.11-1802/ts.201502016
利用啤酒糟制备高品质饲料蛋白
Study on the preparation of high-quality feedstuffs protein by brewer's spent grain
摘要
Abstract
After blade grinding and sieving pretreatment,the partial of brewer' s spent grain with high level of protein was dealt with ball milling,solid enzymatic digestion and fermentation with beneficial microorganism.The feeding quality of brewer' s spent grain has been significantly improved,resulting in the production of feed protein with high-quality.The contents of crude protein and crude fiber in the undersize brewer's spent grain were 38.8％ and 1.89％,respectively after blade grinding and filtration through a 60 mesh sieve.Consequently,the preliminary enrichment and separation of protein and fiber had been achieved.Moreover,the enzymatic efficiency of undersize brewer' s spent grain could be further improved after milling of fiber bundle by ball milling.After the hydrolysis by cellulase,xylanase,protease and solid fermentation with Candida utilis JX-09,the crude protein content of brewer's spent grain was increased up to 45.17％,which was 59.6％ higher than that of original sample.Polypeptides content was increased from 0.68％ to 5.21％,while the in vitro digestibility of proteins was increased from 53.7％ to 61.8％.Total amino acids content was increased up to 42.18％,which was 60％ higher than that of original sample,and the essential amino acids content was reached 17.3％,which was 60％ higher than that of original sample.Crude fiber content was 1.66％ and the degradation rate reached 89％.关键词
啤酒糟/刀片粉碎/球磨/固态酶解/固态发酵Key words
brewer's spent grain/ blade grinding/ ball milling/ solid enzymatic/ solid fermentation引用本文复制引用
蔡国林,张麟,陆健..利用啤酒糟制备高品质饲料蛋白[J].食品与发酵工业,2015,41(2):89-94,6.基金项目
国家863计划2013AA102109 （）
江苏省科技支撑计划BE2012397 （）
江苏省普通高等学校科研成果产业化推进项目JHB2012-26 （）
广东省安全食品精深加工科技创新平台建设2012B091400030 （）
