食品与发酵工业2015，Vol.41Issue(2)：179-183,5.DOI:10.13995/j.cnki.11-1802/ts.201502031
稳定同位素iTRAQ标记/高效液相色谱-串联质谱法测定3种红菇中游离氨基酸
Simultaneous determination of free amino acids in three edible Russula fungi by stable isotope isobaric tage quantitation labeled-LC MS/MS
摘要
Abstract
Free amino acids in three edible Russula fungi were determined with isotope internal standard method by high performance liquid chromatography-mass spectrometry (LC-MS/MS).The main results show that Russula sp.from Tengxian county contains 32 free amino acids,the total content was 15.14 mg/g; Russula sp.from Rongxian county contains 32 free amino acids,the total content was 25.36 mg/g; Russula virescens contains 30 free amino acids,the total content was 23.10 mg/g.Of the free protein amino acids detected,glutamine was the most abundant amino acid followed by glutamic acid,alanine,and proline.GABA was the most abundant non-protein amino acid followed by β-alanine; the others were found in small amounts or traces.The three edible mushrooms contain 18 free protein amino acids and some physiological non-protein amino acids.关键词
红菇/同位素内标法/游离氨基酸Key words
Russula sp./ isotope internal standard method/ free amino acids引用本文复制引用
施文祥,陈新华..稳定同位素iTRAQ标记/高效液相色谱-串联质谱法测定3种红菇中游离氨基酸[J].食品与发酵工业,2015,41(2):179-183,5.基金项目
国家自然科学基金项目(31160313) （31160313）
