食品与发酵工业2015，Vol.41Issue(3)：1-7,7.DOI:10.13995/j.cnki.11-1802/ts.201503001
乳糖诱导纤维二糖差向异构酶的表达及热处理纯化
Expression and thermal-purification of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus induced by lactose
摘要
Abstract
The feasibility of using lactose to replace IPTG as the inducer for expression of CsCE in E.coli BL21 (DE3) was investigated.The induction conditions for CsCE expression were optimized.Final concentration of 10 g/L lactose was added into broth when the cell growth reached OD600 =0.6.After incubation at 25 ℃C for another 20 h,the maximum CsCE activity was approximately 801 U/L,the total activity and the specific activity were 1.4 times and 37％ higher than those induced by IPTG,respectively.In addition,thermal-purification of CsCE was carried out.The results showed that approximately 85.9％ of the soluble protein was removed due to denaturation.The activity recovery was 86.6％ with a specific activity of 5.46 U/mg and a purifying fold of 6.14.The experimental results presented a promising technology to attain high CsCE production on industrial scale.关键词
纤维二糖差向异构酶(CsCE)/乳果糖/乳糖/诱导表达/热处理纯化Key words
cellobiose 2-epimerase (CsCE)/lactulose/lactose/induced expression/thermal-purification引用本文复制引用
汪明明,杨瑞金,华霄,沈秋云,张文斌,赵伟..乳糖诱导纤维二糖差向异构酶的表达及热处理纯化[J].食品与发酵工业,2015,41(3):1-7,7.基金项目
十二五国家科技支撑计划项目(No.2011BAD23B03) （No.2011BAD23B03）
国家自然科学基金面上项目重点项目(No.31230057) （No.31230057）
