食品与发酵工业2015，Vol.41Issue(3)：33-38,6.DOI:10.13995/j.cnki.11-1802/ts.201503006
碱性木聚糖酶在毕赤酵母的表达及酶学性质研究
Expression of an alkali-tolerant xylanase gene in Pichia pastoris and its enzymatic characterization
摘要
Abstract
In this study,xylanase gene (xynAeSC) from Streptomyces chartreusis L1105 without carbohydrate binding domain (CBD) was cloned into expression vector.Recombinant strain (GS115-3-20) was fermented under the optimal condition for enzyme production.The yield of xylanase enzyme was up to 683 ± 9 U/mL.Results showed that recombinant xylanase had the high relative enzyme activity at pH 6.2 ～ 8.6,especially at 7.2.It had the relative activity more than 70％ at 40 ～ 60 ℃2.The optimal temperature was around 70 ℃.It also showed the highest specific activity towards Oat-speh xylan (equal to 259.7％ ± 10.7％ of relative activity towards Birch xylan).It also showed the lowest activity towards sugar cane pulp xylan (only equal to 16.0％ ± 1.5％ of relative activity towards Birch xylan).Its activity and properties had not significantly affected by removing CBD domain.All these study made this expression system attractive for industrial applications.关键词
碱性木聚糖酶/毕赤酵母/纤维素结合域/酶性质Key words
Xylanase/Pichia pastoris/CBD/Properties of enzyme引用本文复制引用
熊科,杨玉焕,闫子祥,王晓玲,李秀婷..碱性木聚糖酶在毕赤酵母的表达及酶学性质研究[J].食品与发酵工业,2015,41(3):33-38,6.基金项目
国家自然科学基金(31371723) （31371723）
北京市自然科学基金(5144026) （5144026）
食品科学创新团队(IDHT20130506) （IDHT20130506）
