食品与发酵工业2015，Vol.41Issue(3)：215-218,4.DOI:10.13995/j.cnki.11-1802/ts.201503038
ATP荧光微生物快检法中不同材质及孔径微孔滤膜细菌截留效果的评价
Evaluation on microporous membrane with different material and aperture through ATP fluorescent rapid detection
摘要
Abstract
The bacterial retention performances of mixed cellulose membrane,polycarbonate membrane,cellulose nitrate membranes and cellulose acetate membranes with 13 mm-diameter were investigated under the same conditions.The total plate count of bacteria in the tested sample was 10 CFU/mL.The filtrate volume was 50 mL,and the filtration was repeated 5 times.When the aperture was 0.22 μm,the average retention rates of four membranes were 100％,94％,84％ and 72％,respectively.When the aperture was 0.45 μm,they were 92％,82％,70％ and 70％,respectively.Therefore,the best membrane to retain bacteria was mixed cellulose membrane with 0.22 μm aperture.50 mL samples respectively containing bacteria 42 CFU/mL,20 CFU/mL,10 CFU/mL and 4 CFU/mL were separately filtered through mixed cellulose membrane.The bacteria on the membranes were determined by ATP bioluminescent method.There was a good linear correlation between fluorescent value and the bacteria concentration(R2 ＞ 0.9).The experiment results proved that mixed cellulose membrane with 0.22 μm aperture could meet the requirements about concentration and measurement of low-level bacteria content sample by ATP bioluminescent method.关键词
微孔滤膜/截留率/菌落总数/ATP生物发光法Key words
microporous membrane/retention rate/total plate count/ATP bioluminescent method引用本文复制引用
侯玉柱,田雨,柯润辉,尹建军,宋全厚..ATP荧光微生物快检法中不同材质及孔径微孔滤膜细菌截留效果的评价[J].食品与发酵工业,2015,41(3):215-218,4.基金项目
“十二五”国家科技支撑计划“食品加工过程质量安全在线监测与控制技术研究示范”课题(No.2012BAO29B04) （No.2012BAO29B04）
