包装与食品机械Issue(3)：67-69,3.DOI:10.3969/j.issn.1005-1295.2014.03.018
HPLC 法测定葛根超细粉中葛根素含量
Studies on the Content Determination of Puerarin in Roots of Puerarin Lobata Superfine Powder by HPLC
任少伟 1张文成 1李兵 1董秀丽 1李如福 2李学彬 2李广龙2
作者信息
- 1. 合肥工业大学农产品生物化工教育部工程研究中心，合肥230009
- 2. 安徽信屹康中药工业有限公司，合肥230001
- 折叠
摘要
Abstract
This study has been to establish a method of determination of puerarin in roots of Puerarin loba -ta superfine powder.SGE protecol C18 (5μm,4.6 ×250mm) chromatographic column was used ,with mix-tures of methanol and water as mobile phase (25∶75,v/v),the flow rate was 1.0mL/min,column temperature was 35℃,the wavelength was 250nm,the injection volume was 20 μL.The linear correlation of puerarin is good in range of 47~470μg/ml,the correlation coefficient r2 is 0.9998.The presented method is simple and reliable for determining of roots of Puerarin lobata superfine powder .关键词
葛根素/超微粉/含量测定Key words
puerarin/superfine powder/content determination分类
轻工纺织引用本文复制引用
任少伟,张文成,李兵,董秀丽,李如福,李学彬,李广龙..HPLC 法测定葛根超细粉中葛根素含量[J].包装与食品机械,2014,(3):67-69,3.
