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高效液相色谱法快速测定莱芜生姜中的姜黄素

曹峰 孙森 亓振 王晓花

包装与食品机械Issue(3):70-72,69,4.
包装与食品机械Issue(3):70-72,69,4.DOI:10.3969/j.issn.1005-1295.2014.03.019

高效液相色谱法快速测定莱芜生姜中的姜黄素

Rapid Determination of Curcumin in Laiwu Ginger by HPLC

曹峰 1孙森 2亓振 1王晓花1

作者信息

  • 1. 莱芜市食品药品检验检测中心,山东莱芜271100
  • 2. 微山县产品质量监督检验所,山东微山277600
  • 折叠

摘要

Abstract

A rapid method for determination of curcumin in Laiwu ginger by HPLC was developed in this paper.This method used water-ethanol(20∶80)solution as extract,and liquid chromatographic separation was performed on a Symmertry Shield RP18 column.The mobile phase was acetonitrile -0.02mol/L ammoninum acetate (60∶40)with column temperature 25℃,and lastly detected at 425 nm through a UV detector.The sep-aration was achieved within 8min,the average recovery 98.2%,RSD2.35%,and detection limits 5.0 ×10 -4 g/kg.

关键词

高效液相色谱法/快速测定/莱芜生姜/姜黄素

Key words

HPLC/rapid determination/Laiwu ginger/curcumin

分类

轻工纺织

引用本文复制引用

曹峰,孙森,亓振,王晓花..高效液相色谱法快速测定莱芜生姜中的姜黄素[J].包装与食品机械,2014,(3):70-72,69,4.

包装与食品机械

OACSTPCD

1005-1295

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