茶叶科学Issue(5)：515-524,10.
不同品种（系）凤凰单丛茶DNA指纹图谱的构建
Construction of DNA Fingerprints in Fenghuang Dancong Tea of Different Cultivars (or Lines)
摘要
Abstract
The aim of this study is to quickly and accurately identify different cultivars (or lines) of Fenghuang Dancong tea in which would construct a DNA fingerprint in different cultivars (or lines) of Fenghuang Dancong tea based on ISSR molecular marker. Firstly, 58 ISSR primers were screened with 4 cultivars, indicating 12 of which are polymorphic. After screening the optimal annealing temperature, 12 ISSR primers were used to amplify 39 Fenghuang Dancong tea resources. The results showed that by using 6 reliable ISSR primers, 84 DNA fragments, among which 78 DNA bands were polymorphic, of all 39 tea accessions were amplified. And the polymo rphic proportion of DNA bands (PPB) was 92.86%, including the primer UBC843 with the richest polymorphic, and the average number of DNA bands amplified by each primer was 14. The average polymorphism information content (PIC) was 0.9456, and the genetic similarity coefficient of the 39 accessions ranged from 0.4063 to 0.9836. In order to identify all 39 accessions, it indicated that the combination efficiency of UBC827/UBC846 was the highest by making combination of the 6 core ISSR primers. By connecting the name of cultivars, the national area code, the sampling sites, the name of the highest efficient primer combination and the ISSR data code, the DNA fingerprint codes of 39 Fenghuang Dancong tea were built. And then, the relevant information of the sample such as its flavor type, altitude, ISSR-PCR amplified picture and so on, were all typed in the software to generate the two-dimensional code fingerprint. The construction of the DNA fingerprint in different cultivars (or lines) of Fenghuang Dancong tea could offer data support for the protection of the cultivars rights and interests.关键词
凤凰单丛茶/ISSR/DNA指纹图谱Key words
Fenghuang Dancong tea/ISSR/DNA fingerprint分类
轻工纺织引用本文复制引用
马瑞君,梅洪娟,庄东红,杨培奎,吴清韩..不同品种（系）凤凰单丛茶DNA指纹图谱的构建[J].茶叶科学,2014,(5):515-524,10.基金项目
广东省科技计划项目（2011B020304012）、广东省高等学校科技创新重点项目 （）
