粮油食品科技Issue(4)：76-81,6.
转基因小麦外源基因插入位点初步分析及检测方法的建立
Preliminary analysis of insertion site of transgenic wheat exogenous gene and the establishment of detection method
摘要
Abstract
The sequence character of insertion site of transgenic wheat exogenous gene sGNA was analyzed by genome -walking and the Nested -PCR methods,and the method of detection of sGNA gene trans-form event specificity was established.The random primers were supplied by Takara and the specific primers were designed based on the sGNA sequence.The boundary sequences of sGNA were cloned by genome -walking,and located on genome A by the homologous recombination.The Ubi promoter and bar gene of the plasmid with exogenous gene sGNA were cut off during the integration process,and two series of NOS genes were remained,the deletion was resulted in 4 500 ~4 520 bp,and insertion of a seg-ment of base sequence was discovered between 4 786 bp to 4810 bp,which resulted the rearrangement of wheat genome sequence.The accuracy of the insertion site information was verified,so it′s effective and feasible of genome -walking for the detection of safety and identification of transgenic wheat.关键词
sGNA/转基因小麦/插入位点/边界序列/检测方法Key words
sGNA/transgenic wheat/insertion site/boundary sequence/detection method分类
农业科技引用本文复制引用
段晓亮,许兰杰,刘志勇,梁荣奇..转基因小麦外源基因插入位点初步分析及检测方法的建立[J].粮油食品科技,2014,(4):76-81,6.基金项目
转基因生物新品种培育重大专项 （）
