粮油食品科技Issue(2)：68-72,5.
两种强启动子在枯草芽孢杆菌中调控表达研究
Study on the expression of regulation of two strong promoters in bacillus subtilis
摘要
Abstract
The effect of two kinds of constitutive strong promoters P43 and PlapS on regulation of heterolo-gous gene was compared on the expression level of ZEN degrading enzyme gene ZLHY6 and the enzyme activity evaluation.The degrading enzyme gene regulated by PlapS received efficient expression in bacillus subtilis 168,and the degrading enzyme activity reached the highest level of 219.02 U/mL after fermentation for 12 h.Moreover,the genetic stability of ZEN degrading enzyme gene expression vector pWBZ7 regulated by PlapS in Bs 168 lays a foundation for efficient expression and secretion of degrading enzyme.关键词
玉米赤霉烯酮/枯草芽孢杆菌/启动子/高效表达系统Key words
zearalenone/bacillus subtilis/promoter/efficient expression system分类
生物科学引用本文复制引用
周艳敬,常晓娇,吴子丹,伍松陵,孙长坡..两种强启动子在枯草芽孢杆菌中调控表达研究[J].粮油食品科技,2015,(2):68-72,5.基金项目
粮食公益性行业科研专项 （）
