摘要
Abstract
A method for determination of albendzole ( ABZ) and its metabolites, albendazole sulfox-ide( ABZSO) , albendazole sulfone ( ABZSO2 ) and albendazole aminosulfone ( ABZ-2-NH2 -SO2 ) in surimi with ultra performance liquid chromatography-tandem mass spectrometry ( UPLC-MS /MS) was established. The surimi samples were extracted by ethyl acetate and cleaned up by n-hexane, and detec-ted by LC-MS/MS. The limit of quantitation ( LOQ) of ABZ was 0. 5μg/kg, and the linear range was 0�5-50 ng/mL, while the LOQ of its metabolites was 0. 1μg/kg, and the linear range was 0. 1-10 ng/mL. When the standard addition level was 0. 5 -5μg/kg, the average recoveries of ABZ was 96. 6% -99. 7%,and the RSD was 5. 95% -9. 95%. When the standard addition level was 0. 1-1μg/kg, the av-erage recoveries of its metabolites was 90. 8% -105. 6%,and the RSD was 2. 16% -9. 15%.关键词
阿苯达唑及其代谢物/超高效液相色谱-串联质谱联用/鱼糜Key words
albendzole and its metabolites/ultra performance liquid chromatography tandem mass spectrometry/surimi
