肉类研究Issue(5)：22-26,5.DOI:10.7506/rlyj1001-8123-201505006
液相色谱串联质谱法测定猪肉中克伦特罗手性对映体残留量
Determination of Residual Clenbuterol Enantiomers in Pork by Liquid Chromatography with Tandem Mass Spectrometry
摘要
Abstract
A method was developed for determining residual clenbuterol enantiomers in pork by liquid chromatography with tandem mass spectrometry. Five grams of samples were extracted with ethyl acetate under basic condition. Then, clenbuterol enantiomers were back-extracted from the extracts using diluted hydrochloric acid. The hydrochloric acid extract was purified by SCX solid phase extraction (SPE) cartridge. The eluent was dried by blowing nitrogen and the residue was dissolved in 500 μL of methanol with 0.02% acetic acid and 0.1% ammonium acetate. The analytes were analyzed by LC-MS/MS on a chiral column (Astec CHIOBIOTICTM V) with a mixture of methanol-acetic acid-ammonium acetate as the mobile phase and quantified by the internal standard calibration curve method. Good linearities were obtained for two enantiomers in the concentration range of 2.0 – 200 μg/L with a correlation coefficient more than 0.997 0. The recoveries for the two enantiomers in pork were 95.8% – 102.1% at the fortified levels of 0.25 – 0.75 μg/kg with relative standard deviations less than 5%. The limits of detection (LOD) and quantitation (LOQ)were 0.10 and 0.25 μg/kg, respectively.关键词
克伦特罗/手性对映体/液相色谱串联质谱法/固相萃取Key words
clenbuterol/enantiomers/liquid chromatography with tandem mass spectrometry/solid phase extraction分类
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陈国,刘勇军,吕燕,孙亚米,吴银良..液相色谱串联质谱法测定猪肉中克伦特罗手性对映体残留量[J].肉类研究,2015,(5):22-26,5.基金项目
宁波市重大科技攻关项目 （）
