中国果菜Issue(12)：30-34,5.
啤酒废酵母细胞破壁方法的研究
Research on the Cell Wall-Breaking Method of Waste Beer Yeast
摘要
Abstract
The methods of repeated freezing and thawing, ultrasonic, microwave, salt, enzymatic and liquid nitrogen grinding on wall-breaking were compared and the trehalose yield and broken walls of time were analyzed in this study. Results showed that the method of microwave was the best way to get the rate of broken yeast cells (49%), the trehalose yield (5.64%) and the time (30min). Through the study of the six wall-breaking methods, we provided experimental basis and technical conditions to further comprehensive exploitation and utilization of waste beer yeast.关键词
啤酒废酵母/破壁方式/革兰氏染色/海藻糖Key words
Beer waste yeast cells/methods of broken wall/gram staining/trehalose分类
生物科学引用本文复制引用
孟国庆,王传宝,朱陶,张海丽,王宜磊..啤酒废酵母细胞破壁方法的研究[J].中国果菜,2014,(12):30-34,5.基金项目
山东省科技计划项目（2011GSF2114）；菏泽市科技计划项目 （）
