中国食物与营养Issue(12)：62-65,4.
槲皮素对前列腺癌PC-3细胞凋亡和HSP27表达的影响
Effect of Quereetin on Prostate Cancer PC-3 Cell Apoptosis and HSP27 Expression
摘要
Abstract
!O!b!jec"tive !!"To observe the effect of quereetin on prostate cancer PC-3 cell apoptosis and heat shock protein 27 (HSP27) expression, and discuss therapeutic mechanism of quereetin. !M!e!tho"d ! !"RT-PCR of the HSP27 was implemented by the culture of RWPE-1, LNCaP, PC-3, and TSU-Pr1 cell lines. Analysis was separately conducted in the control group, control vector group treated by dimethyl sulfoxide (DMSO), and groups treated with 150μL quereetin at 0. 3 or 0. 6mg/mL concentration. PC-3 cell apoptosis was evaluated by TUNEL staining, and the expression of HSP27 was observed in RT-PCR. !!Re!su"lt !!"After quereetin treatment, the number of PC-3 cell apoptosis at 0. 6mg/mL quereetin concentration was stronger compared to that at 0. 3mg/mL concentration. Compared with the control group, the difference was statistically significant (P<0. 05) . Compared with the control group, the HSP27 expression detected by RT-PCR was stronger at 0. 6mg/mL quereetin concentration compared to that at 0. 3mg/mL concentration, and the difference was statistically significant (P<0. 05) . !C!on!cl"usion !!!"Quereetin could induce apoptosis in PC-3 and inhibit HSP27 expression, which might be one mechanism of its anti-prostate cancer.关键词
槲皮素/前列腺癌/热休克蛋白27/细胞凋亡/前列腺癌PC-3细胞Key words
quereetin/prostate cancer/heat shock protein 27 (HSP27)/cell apoptosis/prostate cancer PC-3 cell引用本文复制引用
于峰,邸彦橙,姜丽丽..槲皮素对前列腺癌PC-3细胞凋亡和HSP27表达的影响[J].中国食物与营养,2014,(12):62-65,4.基金项目
黑龙江省卫生厅科研课题“槲皮素对前列腺癌细胞株HSP27表达的影响”(项目编号:2013-265)。 （项目编号:2013-265）
