食品与发酵工业2015，Vol.41Issue(5)：30-34,5.DOI:10.13995/j.cnki.11-1802/ts.201505006
生物柴油副产品粗甘油浓度对裂殖壶菌生长与积累油脂的影响
Effect of concentration of crude glycerol as biodiesel byproduct on the growth and lipid accumulation of Schizochytrium sp.WZU 6961
摘要
Abstract
Based on the optimization of fermentation of oil-producing Schizochytrium sp.WZU 6961 with biodiesel byproduct crude glycerol,the effect of the concentration of crude glycerol on the strain's growth and lipid accumulation was studied.Using the same culture condition including temperature,dissolve-d oxygen,and the same other medium ingredients,the results showed that there was no significant change of the oil content and fatty acid composition with the increase of crude glycerin concentration,while the biomass and oil yields of glycerol,and the productivities of biomass and oil were gradually declined,which meant that high crude glycerol concentration had inhibitory effect on the growth of cells.For crude glycerol concentration of 30 g/L and 60 g/L,biomass productivity was 0.94 g/(L · h) and 0.87 g/(L · h) respectively,and oil productivity was 0.69 g/(L · h) and 0.64 g/(L · h) respectively,which was close to each other in the productivities of biomass and oil.So the suitable crude glycerol concentration was 30g/L.When crude glycerol concentration was 30 g/L,fermentation time was 26h,biomass,oil content and the proportion of DHA in oil were 24.38 ± 1.42g/L,74.08％ ± 2.20％ and 23.72％,respectively.关键词
粗甘油浓度/裂殖壶菌/生物量/油脂含量/脂肪酸组成Key words
crude glycerol concentration/Schizochytrium sp.WZU 6961/biomass/lipid content/composition of fatty acid引用本文复制引用
吴论文,杨美慧,姚瑶,万晓云,周茂洪..生物柴油副产品粗甘油浓度对裂殖壶菌生长与积累油脂的影响[J].食品与发酵工业,2015,41(5):30-34,5.基金项目
浙江省大学生科技创新项目(KZ1312037) （KZ1312037）
