食品与发酵工业2015，Vol.41Issue(5)：41-47,7.DOI:10.13995/j.cnki.11-1802/ts.201505008
重组蔗糖异构酶的制备及应用条件优化
Studies on the preparation of sucrose isomerase and its application for the production of isomaltulose
摘要
Abstract
Based on the study of enzyme production from recombinant strain E.coli BL21 (DE3)/pET-24a-palI fermented in shake flasks,and the effect of different induction concentrations of lactose used in 3 liter fermentor on its production were investigated.Results showed that the activity in the supernatant of shake flasks and 3 liter fermentor (under 0.4 g/(L · h) lactose induction) was 253.1 and 654 U/mL,respectively.Furthermore,the reaction conditions for isomaltulose production by sucrose isomerase were optimized in this study.The optimized conditions were as follows:400 g/L sucrose,pH 6.5,20 U sucrose isomerase per gram sucrose,8 h of incubation at 30 ℃.Under the optimized conditions,the maximal yields of isomaltulose could reach 87.9％.关键词
蔗糖异构酶/异麦芽酮糖/酶转化/摇瓶发酵/高密度发酵Key words
sucrose isomerase/isomaltulose/enzymatic conversion/shake flasks fermentation, liter fermentor引用本文复制引用
程胜,段绪果,吴敬..重组蔗糖异构酶的制备及应用条件优化[J].食品与发酵工业,2015,41(5):41-47,7.基金项目
国家自然科学基金(31425020) （31425020）
863计划(2012AA021501) （2012AA021501）
江苏省自然科学基金(BK20140142) （BK20140142）
