食品与发酵工业2015，Vol.41Issue(5)：66-71,6.DOI:10.13995/j.cnki.11-1802/ts.201505012
乳酸菌及葡萄糖对鱼片中细菌群落及TVBN值的影响
Influence of lactic acid bacteria and glucose on bacterial communities and TVBN value in fillets
摘要
Abstract
In this paper,the influences of lactic acid bacteria and glucose on bacterial communities and volatile base nitrogen (TVBN) value in tilapia fillets were investigated.Results showed that the sensory properties of the fillets hardly changed after the treatment of Lactobacillus acidophilus CICC6074 or 1.5％ glucose solution.The TVBN value of the control (the distilled water treated fillets) exceeded the limit of Chinese National Standard after only 6 d storage at 4 ℃,however,that of the CICC6074 treated fillets didn't reached the above limit until after 12 d storage.The adding of glucose facilitated the growth of bacteria at the earlier stage of cold storage,but didn' t increase the final bacterial population in fillets.However,the added glucose obviously reduced the TVBN level of fillets.Whether the combination of CICC6074 and glucose may lower the TVBN value of fillets more needs to be further investigated.PCR-DGGE analysis showed that in CICC6074 treated fillets,Pseudomonas,the recognized specific spoilage organism f fish,was effectively inhibited,and didn't obviously multiplied until after 6 d storage.However,in the control Pseudomonas,the recognized specific spoilage organism of fish obviously multiplied after only 3 d storage.The population of CICC6074 adsorbed in fillets slightly increased at the early stage,and began to decrease 6 d later and finally disappeared after 9 d storage.These facts illustrate that L.acidophilus CICC6074 is competent in extending the shelf life of fillets,and glucose may reduce the level of TVBN value.关键词
乳酸菌/葡萄糖/鱼片/TVBN/保鲜/DGGEKey words
lactic acid bacteria/glucose/fillet/TVBN/preservation/DGGE引用本文复制引用
周幸芝,段杉,孟晓华,梁少雅,吕建秋,蒋艳萍..乳酸菌及葡萄糖对鱼片中细菌群落及TVBN值的影响[J].食品与发酵工业,2015,41(5):66-71,6.基金项目
广东省科技计划项目(2012B040400004)“珠海市白蕉专业镇科技服务对接平台建设” （2012B040400004）
广东省海洋渔业科技推广专项(A201353C05)“高品质罗非鱼片生产及废弃物高值化利用研究及产业化示范” （A201353C05）
粤港关键领域重点突破项目(2010A020104004) （2010A020104004）
