茶叶科学Issue(4)：307-315,9.
茶尺蠖类免疫球蛋白基因 EoHML 的克隆和表达分析
Molecular Cloning and Expression Analysis of Hemolin Gene in Tea Geometrid (Ectropis obliqua)
摘要
Abstract
Hemolin, a unique immune protein, belongs to the immunoglobulin superfamily of Lepidoptera insects. A full length cDNA sequence of hemolin gene was cloned by RACE-PCR from tea geometrid (Ectropis obliqua Prout), which was named as EoHML (GenBank Accession No. KM885983). The results of bioinformatics characterization showed that the full length cDNA of EoHML was 1 772 bp, which contained an intact CDS of 1 239 bp and encoded 412 amino acid residues with a putative molecular mass of 45.8 kD and an isoionic point of 8.297. The deduced amino acid sequence demonstrates it belongs to a typical secretory protein that contains 4 Ig functional conservative areas and 2 N-glycosylation sites. Phylogenetic analysis showed that EoHML share the far genetic distance with published hemolin gene and the highest similarity with that of Manduca sexta was only 53%. The results of quantitative real-time PCR analysis further revealed a significant up-regulated expression about 36.5 times in the larvae infected E.obliqua Nucleopolyhedrovirus (EoNPV) than the controls. The study indicated that the EoHML gene may participate in the immune response to EoNPV in tea geometrid.关键词
茶尺蠖/类免疫球蛋白/基因克隆/表达分析Key words
tea geometrid (Ectropis obliqua)/hemolin/gene cloning/expression analysis分类
农业科技引用本文复制引用
余玉庚,袁志军,殷坤山,付建玉,肖强..茶尺蠖类免疫球蛋白基因 EoHML 的克隆和表达分析[J].茶叶科学,2015,(4):307-315,9.基金项目
中央级公益性科研院所基本科研业务专项（0032014014）、科技基础性工作专项 （）
