茶叶科学Issue(4):347-353,7.
千家寨不同海拔野生茶树的 EST-SSR 遗传多样性研究
Genetic Diversity of Wild Tea Plant in Different Altitude in Qianjiazhai
摘要
Abstract
5 pairs of EST-SSR markers derived by the authors were used to study genetic diversity and genetic structure of 7 wild tea populations at different altitudes in Qianjiazhai. At the species level, Shannon information index (I) and Nei’s gene diversity (He) were 1.33 and 0.66, respectively, indicating this species has high genetic diversity at species level. Along altitudinal gradients, the genetic diversity among population of wild tea was different and showed low-high-low distribution. The genetic diversity of population at altitude 2 100 m was much higher than others populations. AMOVA analysis showed that only 16.32% of the total genetic variation occurred among populations, whereas 83.68% of the variance was within populations, which was in line with the coefficient of genetic differentiation (Fst = 0.12). And the gene flow Nm was 1.84, indicating the genetic diversity between wild ancient population in was characterized by the moderate level. The genetic characteristics of wild tea and the habitat heterogeneity in different elevation are the main reason of existing genetic pattern.关键词
野生茶树/海拔梯度/遗传多样性/遗传分化/EST-SSRKey words
wild tea/altitude/genetic diversity/genetic differentiation/EST-SSR分类
农业科技引用本文复制引用
黄晓霞,唐探,姜永雷,冯程程,程小毛..千家寨不同海拔野生茶树的 EST-SSR 遗传多样性研究[J].茶叶科学,2015,(4):347-353,7.基金项目
国家自然科学青年基金项目(31100292)、西南林业大学科研启动金项目(110909)、云南省省级重点学科园林植物与观赏园艺建设经费项目 ()