肉类研究Issue(7)：20-24,5.DOI:10.7506/rlyj1001-8123-201507005
乌珠穆沁羊生长过程中骨骼肌MyHC基因变化
Changes in Myosin Heavy Chain (MyHC) Gene from Skeletal Muscle during the Growth of Wuzhumuqin Sheep
摘要
Abstract
This study was aimed to investigate the changes in the relative expression level of myosin heavy chain (MyHC) gene from skeletal muscle during the growth of Wuzhumuqin sheep. Free-grazing Wuzhumuqin sheep were slaughtered at 1, 3, 6, 9, 12 and 18 months of age and thebiceps femoris andlongissimus dorsi muscles were excised. MyHC isoforms were separated from the muscles by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the relative expression level ofMyHC gene was measured by real-time polymerase chain reaction (PCR). The results showed that four clear bands representing typeⅡa, typeⅡx, typeⅡb and typeⅠ, respectively, were observed in the electrophoretic pattern. The expression ofMyHC typeⅠ gene and typeⅡa inbiceps femoris andlongissimus dorsiwas higher than that ofMyHC typeⅡb gene. Understanding theMyHC gene expression in skeletal muscle during the growth of sheep is of great practical importance for improving meat quality and post-mortem physiological and biochemical characteristics.关键词
乌珠穆沁羊/骨骼肌/肌球蛋白重链基因/实时荧光定量聚合酶链式反应Key words
Wuzhumuqin sheep/skeletal muscle/myosin heavy chain/real time-polymerase chain reaction (PCR)分类
轻工纺织引用本文复制引用
冯青辉,斯琴其木格,苏和,朝格赛,薛宝玲,格日勒图..乌珠穆沁羊生长过程中骨骼肌MyHC基因变化[J].肉类研究,2015,(7):20-24,5.基金项目
国家自然科学基金地区科学基金项目(31360368)；内蒙古草原英才基金项目 （31360368）
