食品与发酵工业2015，Vol.41Issue(7)：24-28,5.DOI:10.13995/j.cnki.11-1802/ts.201507005
重组大肠杆菌产普鲁兰酶发酵条件优化
Optimization of fermentation conditions for pullulanase production by recombinant Escherichia coli
摘要
Abstract
By using auto-induction system,the fermentation conditions including oxygen and pH were controlled in a 7-L batch system,and then fed-batch fermentations were carried out using glycerol as a carbon source and lactose as inducer so as to discuss the effects on fermentation by different feeding modes.The optimum conditions were found to be as follows:the dissolved oxygen concentration was maintained at 20％ ～30％,the culture pH was kept at 7.60,and glycerol was added by the rate of 0.8 g/(L · h) at 9h,lactose was added by the velocity of 0.2 g/(L · h) at 20 h.Using the optimal conditions,concentration of cells and activity of pullulanase were increased from 12.58 to 42.25 and from 356 U/mL to 1200 U/mL respectively,which were 3.36 and 3.37 times of those in fed-batch fermentation.关键词
普鲁兰酶/发酵优化/分批补料发酵/恒速流加/自诱导培养/DO-statKey words
pullulanase/optimization/fed-batch fermentation/constant feeding/auto-induction/DO-stat引用本文复制引用
王小兰,穆晓清,徐岩,聂尧..重组大肠杆菌产普鲁兰酶发酵条件优化[J].食品与发酵工业,2015,41(7):24-28,5.基金项目
国家重点基础研究发展(973)计划项目(2011CB710800) （973）
国家高技术研究发展(863)计划项目(2012AA022207) （863）
高等学校学科创新引智计划项目(111计划,111-2-06) （111计划,111-2-06）
国家自然科学基金(21176103) （21176103）
高端外国专家项目(GDW20133200113) （GDW20133200113）
