食品与发酵工业2015，Vol.41Issue(7)：45-50,6.DOI:10.13995/j.cnki.11-1802/ts.201507009
天山一号冰川融水中耐冷酵母菌多样性及系统发育
Diversity and phylogeny of cold-adapted yeast from melt-water in the No.1 Glacier, Tianshan Mountains
摘要
Abstract
Theculturable yeast from melt-water of the Glacier No.1 in the Tianshan Mountains,were isolated and screened.The growth characteristics,system development diversity of psychrophilic yeast and microbial species diversity in the glacial environment were investigated,which laid the foundation for collection and protection of psychrophilic yeast resources and development of its related potential biological technology.The screening media of RB was used to isolate yeast strains.The optimum growth temperature,growth pH and salt resistance on the separation of the strains were tested.The productions of extracellular enzymes from strains were respectively detected through selective medium.26S rRNA gene sequence analysis was used to determine system evolution status of psychrophilic yeast.The strains with highly homologous 26S rRNA genes were further distinguished through the MSP-PCR fingerprint technology.Of the total 68 strains of yeast isolates,15 representative strains were selected according to the initial clustering of colony morphology and cell morphology and were sequenced.The results showed that these 15 yeasts strains respectively belonged to 7 genera of basidiomycotina and ascomycetes,only BCY-12 belonged to ascomycetes of Pichia genera,other all belong to basidiomycotina,wherein the cryptococcus accounted for one third of the total.Research on enzyme production traits showed that 7 strains could produce lipase,4 strains could produce lipases,11 strains could produce amylase,11 strains could produce protease,3 stains could produce pectinase,and 2 strains could produce chitinase.The psychrophilic yeasts were abundant in the Glacier.Phylogenetic diversity was obvious and enzyme production characteristic was different.According to the growth temperature,most isolates belonged to the cold resistant strains,wherein strains of the Cryptococcus genus were dominant population.关键词
天山冰川/耐冷酵母菌/多样性/系统发育/产酶性状Key words
Tianshan Mountains/psychrophilic yeast/diversity/phylogeny/enzyme production characteristics引用本文复制引用
郑晓吉,孙海龙,关波,姜远丽,倪永清..天山一号冰川融水中耐冷酵母菌多样性及系统发育[J].食品与发酵工业,2015,41(7):45-50,6.基金项目
国家自然科学基金项目:天山一号冰川底部沉积层及其前沿退缩带微生物群落时空演替格局对环境分异的响应(41271268) （41271268）
