食品与发酵工业2015，Vol.41Issue(7)：149-154,6.DOI:10.13995/j.cnki.11-1802/ts.201507027
乳品中3种常见细菌多重PCR检测方法的建立
Establishment of multiple PCR for detection of three kinds of common bacteria in dairy products
摘要
Abstract
The genome DNAs of Escherichia coli,Staphylococcus aureus and Salmonella were extracted.The pho A gene of Escherichia coli,nuc A gene of Staphylococcus and inv A gene of Salmonella were used as the target genes.A multiplex polymerase chain reaction assay for simultaneous identification of three pathogenic bacteria was established.In contrast to national standard methods,the sensitivity and specificity of multiplex PCR in detecting three bacteria from dairy products were studied.The results showed that the detection results by multiplex PCR method were the same as national standard methods.The results were stable in simulated examination.The multiplex PCR method was more rapid,sensitive and specific than national standard method.The established multiplex PCR method could provide the scientific evidence in detection of pathogenic bacteria from dairy products.关键词
乳品/多重PCR/大肠杆菌/金黄色葡萄球菌/沙门氏菌Key words
dairy/multiplex PCR/Escherichia coli/Staphylococcus aureus/Salmonella引用本文复制引用
闫晗,吕国钦,张洋,张笑嫣,冯旭,王丽威..乳品中3种常见细菌多重PCR检测方法的建立[J].食品与发酵工业,2015,41(7):149-154,6.基金项目
国家自然科学基金面上项目(31270479) （31270479）
