食品与发酵工业2015，Vol.41Issue(7)：256-262,7.DOI:10.13995/j.cnki.11-1802/ts.201507047
不同提取方法对山药多糖含量及其体外抗氧化活性的影响
Effect of extraction methods on the yield and in vitro antioxidant capacity of polysaccharides from Chinese yam (Dioscorea opposita Thunb.)
摘要
Abstract
Polysaccharides from Chinese yam (Dioscorea opposita Thunb.) were extracted with hot water,ultrasonic,microwave and enzymes respectively,and content was determined by phenol-sulfuric acid.Four assay methods for antioxidant capacity in vitro including reducing power,hydroxyl radical assay,DPPH · scavenge activity and superoxide radical assay were applied.The results showed that enzymes extraction methods presented the highest yield,9.65％ and a short extraction time of 120 min.In terms of reducing power,hydroxyl radical assay and DPPH · scavenge activity,enzymes extraction showed the best antioxidant capacity,followed by microwave,hot water and ultrasonic methods.As to the superoxide radical assay,microwave extraction methods was the best when polysaccharides concentration was above 2 mg/ml.Overall,enzymatic methods is better than other methods in improving yield and maintaining the functional activities.Enzyme method also significantly decreases the extraction time and is more safe and environment friendly.关键词
山药/多糖/提取/抗氧化Key words
Chinese yam/dioscorea opposita/polysaccharides/extraction/antioxidant capacity引用本文复制引用
高行恩,王洪新..不同提取方法对山药多糖含量及其体外抗氧化活性的影响[J].食品与发酵工业,2015,41(7):256-262,7.基金项目
淮安市食品技术研究院HAP201301 （）
江苏省高等职业院校国内高级访问学者计划资助项目(2013) （2013）
