茶叶科学Issue(1)：55-63,9.
茶树GDP-D-甘露糖焦磷酸化酶基因cDNA全长的克隆与表达分析
Cloning and Expression Analysis of GDP-D-mannose PyrophosphorylasecDNA inCamellia sinensis
摘要
Abstract
Ascorbic acid(Vitamin C, Vc) is an important antioxidant in tea plant, playing important roles in metabolism and responses to abiotic stress, and tea qualityis positive correlation with the content ofVc.GDP-D-mannosepyrophosphorylase(GMP) is an important enzyme in the synthesis of ascorbic acid.The full-length cDNA sequence ofGMPgene was isolated from the shoots ofCamellia sinensisby RT-PCR and RACE. The entire GMPcDNAwas 1510bp, containing a 1086bp complete open reading frame which encoding a protein with 361 amino acidsand a calculated molecular weight of39.599kDa.Blastanalysis showedthatGMPgene in Camellia sinensiswas most closely toActinidiawith 96% amino acids similarity.Quantitative real-time PCR analysis showed that the expression levels ofGMPgene in the third leaves were highest while the stem is the lowest, and the different varieties also existed an obvious differences. High temperature stress stimulate the expression of GMPgene and the accumulation ofVcin incipient stage, then reduced rapidly.关键词
茶树/GDP-D-甘露糖焦磷酸化酶/基因克隆/表达分析Key words
Camellia sinensis/GDP-D-mannosepyrophosphorylase(GMP)/geneclonging/expression analysis分类
轻工纺织引用本文复制引用
肖瑶,周天山,李佼,张佳欣,余有本..茶树GDP-D-甘露糖焦磷酸化酶基因cDNA全长的克隆与表达分析[J].茶叶科学,2015,(1):55-63,9.基金项目
中央高校基本科研业务费专项（QN 2013017）、唐仲英育种基金项目（10YZ034）、西北农林科技大学农业科技推广专项 （）
