茶叶科学Issue(3)：237-241,5.
高效液相色谱柱后光化学反应-荧光检测茶叶中黄曲霉毒素 B1
Determination of Aflatoxins B1 in Tea by High Performance Liquid Chromatography-Fluorescence Detector with Post-column Photochemical Reaction
赵浩军 1王坤 1杨卫花 1杨朝义1
作者信息
- 1. 大理州质量技术监督综合检测中心，云南 大理 671000
- 折叠
摘要
Abstract
A new method for the sensitive determination of Aflatoxins B1 in tea by high performance liquid chromatography with photoelectric reactor and fluorescence detector was established. A solution of V(acetonitrile)∶V(H2O)=86∶14 was used to extract Aflatoxins B1 from tea. The extracted solution was then purified by a multifunctional and immuneaffinity column, respectively. The peak area and the concentration of Aflatoxins B1 showed a good linear relationship within the range from 0.591 μg/L to 5.91 μg/L with a linear correlation coefficient (r) of 0.9994. The recoveries at the concentrations studied [low level (7.090 μg/kg), high level (14.180 μg/kg)] were between 85.6% and 98.9% with a relative standard deviations ranging from 1.7% to 1.8%. The limit of detection (LOD) is 0.1 μg/kg (S/N=3). The limit of quantitation (LOQ) is (0.591 μg/kg). The new method was used to analyze eight tea samples collected from the local markets and negative results were obtained. The method is suitable for detection of Aflatoxins B1 in tea with high selectivity and sensitivity.关键词
黄曲霉毒素 B1/茶叶/高效液相色谱/多功能净化柱/免疫亲和柱/光化学反应器Key words
Aflatoxins B1/tea/high performance liquid chromatography/multifunctional purifying column/immunoaffinity column/photochemical reactor分类
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赵浩军,王坤,杨卫花,杨朝义..高效液相色谱柱后光化学反应-荧光检测茶叶中黄曲霉毒素 B1[J].茶叶科学,2013,(3):237-241,5.
