茶叶科学Issue(5)：449-457,9.
茶丽纹象甲白僵菌广东分离株的鉴定及生物学特性研究
Identification and Biology Characteristics Research of a Myllocerinus aurolineatus-BeauveriaStrain Isolated from Guangdong Province
摘要
Abstract
Through morphological characters and rDNA-ITS identification, aMyllocerinus aurolineatus-Beauveria strain that was isolated from tea plantation in Yingde Guangdong Province was identified to beBeauveria bassiana, and named as Bb1-1. In order to confirm the biocontrol potential of Bb1-1, two highly virulentB. bassiana strain towardsM. aurolineatusweevil used as the reference, the strain growth rate, sporulation, spore germination rate, thermotolerance and UV resistance of Bb1-1, and the virulence of Bb1-1 toM. aurolineatus weevil under 25℃, 29℃ and 32℃ were tested. The results showed that there were no significant difference on the strain growth rate, spore germination rate, and UV resistance among three strains; the sporulation decreased in this order: XJBb3005> Bb1-1> XJBb3008; however, Bb1-1 showed the best thermotolerance. At 25℃, the cumulative corrective mortality of three strains were all beyond 93.94%, and there were no significance among them. However, the virulence of Bb1-1 toM. aurolineatusweevilwas higher than that of the two reference strains under high temperature. At 29℃, the cumulative corrective mortality was 90.91% (7 d), LT50 value was 3.83d. At 32℃, the cumulative corrective mortality was 80% (7 d), LT50value was 5.28 d. In consideration of Bb1-1’s good thermotolerance and the highly virulent to M. aurolineatus weevil under high temperature, it’ll play a very important role in biocontrol ofM. aurolineatusweevil.关键词
茶丽纹象甲/白僵菌/广东分离株/鉴定/生物学特性/毒力Key words
Myllocerinus aurolineatus/Beauveria/Guangdong strain/identification/biology characteristics/virulence分类
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王定锋,黎健龙,李慧玲,李良德,王庆森,吴光远..茶丽纹象甲白僵菌广东分离株的鉴定及生物学特性研究[J].茶叶科学,2015,(5):449-457,9.基金项目
国家茶叶产业技术体系项目（CARS-23）、中国乌龙茶产业协同创新中心（培育）专项（2013-51）、福建省农业科学院创新团队项目（CXTD-1-1302）、福建省农业科学院茶叶研究所重点项目（2014-cys-02）。 （）
