茶叶科学Issue(5)：481-490,10.
茶树CsbHLH2基因克隆及表达分析
Cloning and Expression Analysis of CsbHLH2 in Tea Plant (Camellia sinensis)
摘要
Abstract
The bHLH(basic Helix-Loop-Helix) transcription factor is one of the most important transcription factor in plants, which playimportant rolesin plant growth andstressregulation. ThebHLH transcription factorCsbHLH2 wascloned fromtea plant (Camellia sinensis) cultivars‘Shanchayihao’byhomologous cloningtechnologyusing cDNA template. Bioinformatics analysis showed thattheCsbHLH2contains714bpORFandwas predictedencoding 297amino acid,the deduced protein molecular weight was58.4kD and its theoretical isoelectric point was5.14. Amino acid sequence alignment revealed thatCsbHLH2was highlyhomologous to other higher plantbHLH proteins. Transient expression of recombinant plasmidCsbHLH2/PBI221-GFPinArabidopsisprotoplasts showed that CsbHLH2was located in cell nuclei.Quantitative real-time PCR analysis of the expression profiles showed that the CsbHLH2gene wasexpressed in bud, leaf,stem and root.The highest expression level of theCsbHLH2wasfound in the youngleaf.Different hormone treatmentsresults showedthattheCsbHLH2was induced by ETH, MEJA and SA treatment, respectively.关键词
茶树/bHLH转录因子/亚细胞定位/进化分析/定量表达分析Key words
Camellia sinensis(L.)/bHLH transcription factor/subcelleular locatlization/phylogenetic analysis/expression analysis分类
农业科技引用本文复制引用
韩永涛,肖斌,钱文俊,梁少茹..茶树CsbHLH2基因克隆及表达分析[J].茶叶科学,2015,(5):481-490,10.基金项目
国家茶叶产业技术体系（CARS-23）、陕西省科技统筹创新 （）
