茶叶科学Issue(5)：501-511,11.
茶树CsCDF1基因克隆及表达分析
Cloning and Expression Analysis of CsCDF1 (Cycling Dof Factor 1) Gene in Tea Plant (Camellia sinensis)
摘要
Abstract
The full-length cDNA of the firstDofgene (CsCDF1)was cloned from tea plant [Camellia sinensis(L.) O. Kuntze] by SMART-RACE cloning technology, and the bioinformatic analysis of the cloned gene were conducted by using online service.The expression profile of this gene in various tissues and with diurnal rhythm as well as in response to different dosage nitrogen treatment were investigated by using real-time fluorescent quantitative RT- PCR. The obtained cDNA sequence was 1 606 bp and contained a complete open reading frame encoding 464 amino acid residues with highly conserved DOF domain. The molecular weight and theoretical isoelectric point (PI) is 50.8 kDa and 5.52 respectively. The analysis by utilizing the BLAST software showed that the derived protein sequences shared separately 69%, 67% and 68% similarity with CDF (Cycling dof factor) proteins inNicotianato-mentosiformis, Solanum tuberosum andNicotiana sylvestris. Phylogenetic analysis showed that the protein encoded by this gene was clustered into the same clade withArabidopsis thaliana CDF, so it was named asCsCDF1. The expression ofCsCDF1 dominated in roots rather than in one bud and two leaves or mature leaves in three phenotypes. During one day,CsCDF1gene changed with the diurnal rhythm, and resupplying nitrogen after starvation for 2 weeks, the expressionin response to different concentration of nitrogen was all raised in different tissues.关键词
茶树/Dof基因/CsCDF1基因/光调控/氮素/表达分析Key words
tea plant (Camellia sinensis)/Dof gene/CsCDF1 gene/light regulation/nitrogen/expression analysis分类
农业科技引用本文复制引用
胡娟,王丽鸳,韦康,成浩,张成才,张芬,吴立赟..茶树CsCDF1基因克隆及表达分析[J].茶叶科学,2015,(5):501-511,11.基金项目
浙江省茶树农业新品种选育重大科技专项（2012C12905）、国家茶叶产业技术体系（nycytx-23）。 （）
