粮油食品科技Issue(5)：45-49,5.
蜂胶中黄酮的提取及其自由基清除活性研究
Study on extraction of flavonoids from propolis and the activity of scavenging free radicals
摘要
Abstract
Taking propolis as raw material,based on single factor experiments,orthogonal test was used to optimize the ultrasonic -assisted extraction of flavonoids,and antioxidant activity of the total flavonoids was studied.The results showed that the order of factors affecting the extraction was ratio of solid to liquid>ethanol concentration >extraction time >extraction temperature;the optimum extraction technology was A2 B2 C2 D2 ,namely ratio of solid to liquid 1∶30,ethanol concentration 80%,extraction time 20 min,ex-traction temperature 50 ℃,under the condition the extraction rate of flavonoids in propolis was 9.60%. Propolis flavonoids had obvious inhibition effect on hydroxy radical and superoxide anion free radical,with obvious dose effect relationship,namely the ability of eliminating free radical strengthened along with the increasing of ethanolic extract density.Flavonoids of propolis with addition amount of 0.15 mg/mL had good antioxidant activity in scavenging free radicals,which was better than natural antioxidant Vc.关键词
蜂胶/黄酮类化合物/超声提取/抗氧化Key words
propolis/flavonoids/ultrasonic extraction/antioxidation分类
轻工纺织引用本文复制引用
曹小燕,杨海涛..蜂胶中黄酮的提取及其自由基清除活性研究[J].粮油食品科技,2015,(5):45-49,5.基金项目
陕西省教育厅专项科研计划（2013JK0680）；陕西理工学院2013年校级人才启动项目 （）
