食品与发酵工业2015，Vol.41Issue(9)：19-23,5.DOI:10.13995/j.cnki.11-1802/ts.201509004
冰亲和吸附装置对胶原抗冻肽的分离纯化
Purification of antifreeze collagen peptides by ice affinity adsorption protocol
摘要
Abstract
Antifreeze collagen peptides is a family of peptides which can lowering the freezing point of the system non-colligatively.This plays an important role in protecting organisms from freezing injury and damage.In this study, we studied the ice affinity adsorption equipment, used micro-organisms protection as the index to optimize the condition of purifying pig skin collagen peptides by enzymatic hydrolysis.The optimum conditions were: crude mixture concentration of 1 mg/mL, at-5 ℃ for 10 hour, adsorption twice.After ice affinity adsorption, collagen peptides peaks had better separation effect, the molecular weight mainly distributed within the range of 1000 Da, and major collagen amino acids were Proline and Hydroxyproline.关键词
胶原酶解复合物/胶原抗冻肽/吸附/冰亲和吸附装置/分子质量分布Key words
collagen hydrolysates/antifreeze collagen peptides/adsorption/ice affinity adsorption protocol/molecular mass distribution引用本文复制引用
阮功成,曹慧,徐斐,于劲松..冰亲和吸附装置对胶原抗冻肽的分离纯化[J].食品与发酵工业,2015,41(9):19-23,5.基金项目
国家自然科学基金青年基金(31201421) （31201421）
国家高技术研究发展计划(863计划)项目(2013AA102207) （863计划）
