食品与发酵工业2015，Vol.41Issue(9)：40-44,5.DOI:10.13995/j.cnki.11-1802/ts.201509008
产普鲁兰酶海单胞菌的分离鉴定及发酵条件优化
Isolation and identification of pullulanase producing strain and optimization of its fermentation conditions
摘要
Abstract
A novel pullulanase-producing strain YH-6 was isolated from the salt lake using pullulan as the sole carbon source.Based on the morphological observation and 16S rRNA sequence analysis, the strain was identified as Oceanimonas sp.The optimum fermentation medium was composed of 15 g/L corn starch, 10 g/L yeast extract, 1.0 g/L KH2PO4, 0.01 g/L FesO4 · 7H2O, 26.5 g/L NaCl, 2.1 g/L MgCl2 · 7H2O, 0.9 g/L KCl, 1.2 g/L CaCl2 · H2O, 4.2 g/L MgSO4 · 7H2O.Under the optimum culture conditions of initial pH 7.0, 30 ℃ and shaking speed of 200 r/min for 24 h, the highest enzyme activity of pullulanase could reach 1.85 U/mL, which was improved by 44.53％.关键词
普鲁兰酶/海单胞菌(Oceanimonas sp.)/鉴定/发酵条件Key words
pullulanase/oceanimonas/isolation/identification/culture conditions引用本文复制引用
严芬,杨光,连燕萍,王培松,吴晨烁..产普鲁兰酶海单胞菌的分离鉴定及发酵条件优化[J].食品与发酵工业,2015,41(9):40-44,5.基金项目
海洋公益性行业科研专项(201305015) （201305015）
