食品与发酵工业2015，Vol.41Issue(9)：45-50,6.DOI:10.13995/j.cnki.11-1802/ts.201509009
1株真菌漆酶高产菌株的筛选及其漆酶的纯化和性质研究
Isolation of a high-level laccase-producing fungus strain, and purification and characterization of laccase
摘要
Abstract
This study aimed to select high-level laccase-producing fungus strain from natural samples.The laccase producing ability of the selected strain and the properties of produced laccase were further studied.The target strain was selected and purified by guaiacol-containing selecting medium and the isolated strain was identified as Trametes sp.by morphology observation and 5.8S rDNA-ITS sequence analysis method.In shaking flask fermentation, a high laccase producing level of 20.3 u/mL was observed in the supernatant on 10t d.The produced laccase protein was salted out by ammonium sulfate fraction and further purified by DEAE FF chromatographic column.The molecular mass of the purified laccase was around 65 kDa, and the laccase was stable under 60 ℃ and pH 3.5 ～ 5.0.The Vmax and Km of the laccase were 31.06 mmol/(L · min) and 152 μmol/L, respectively.The isolated fungus strain Trametes sp.has an ability to produce high-level laccase in a short period, which could make it a good candidate for breeding and optimization of industrial laccase producing strain.关键词
真菌漆酶/筛选/发酵/纯化/酶学性质Key words
fungal laccace/isolation/fermentation/purification/enzyme properties引用本文复制引用
李松,刘宇,汤文晶,陈阿娜,汤斌,崔凤杰,赵鹏翔..1株真菌漆酶高产菌株的筛选及其漆酶的纯化和性质研究[J].食品与发酵工业,2015,41(9):45-50,6.基金项目
安徽省自然科学青年基金资助项目(1408085QC61) （1408085QC61）
安徽上程大学引进人才科研启动基金(2013YQQ003) （2013YQQ003）
国家电网公司科技项目资助(合同号:SGECS56-2014) （合同号:SGECS56-2014）
