茶叶科学Issue(6)：534-542,9.
茶多酚诱导铜绿假单胞菌交叉耐受性研究
Cross-resistance Induced by Tea Polyphenols in Pseudomonas aeruginosa
摘要
Abstract
Cross-resistance induced by sublethal concentration oftea polyphenols(TP)to Pseudomonas aeruginosa was investigated.One-hour exposure of Pseudomonas aeruginosato 1mg·mL-1TP significantly increased the tolerance to multiple environmental stresses, including oxidant(1mmol·L-1H2O2), high temperature (47℃) andacid solutions[phosphate buffer (pH4.0),phosphate buffer (pH4.0) containing 60mmol·L-1citric acid, 60mmol·L-1 lactic acid and 80mmol·L-1acetic acid]. In addition,the effects of TP on expression levels of a few stress-related geneswere studiedby quantitative real-time RT-PCR analysis.It was observed that the expression levels of a few oxidativestress-related geneskat B,sod M,ohr,lex Aandrec Nwere up-regulated by TP, as well as someheat shock protein genes, includingdnaK,groEL,htpG,grpEandgroES.The expression ofthesegenes might play important roles in the development of stress cross-resistance.Although TP was considered to be safe as food preservatives,the underlyinghazardsassociated withcross-resistance inducedby TP should be taken into account in food technology.关键词
茶多酚/铜绿假单胞菌/交叉耐受性/氧化胁迫相关基因/热激蛋白基因Key words
tea polyphenols/Pseudomonas aeruginosa/cross-resistance oxidative stress-related genes/heatshock protein genes分类
轻工纺织引用本文复制引用
刘小香,孙爱华,杜蓬,陈文虎,朱军莉..茶多酚诱导铜绿假单胞菌交叉耐受性研究[J].茶叶科学,2015,(6):534-542,9.基金项目
国家自然科学基金（No.31501581）、浙江医学高等专科学校科研基金项目（No.2013B03）、国家自然科学基金 （）
