沈阳农业大学学报Issue(6)：36-41,6.DOI:10.3969/j.issn.1000-1700.2015.06.006
地被菊‘中国红’再生及遗传转化体系的建立
Establishment of Receptor and Genetic Transformation System of Ground-cover Chrysanthemum'China Red'
摘要
Abstract
Different plant growth regulators that affect adventitious buds induction rate were studied in regeneration system of ground-cover chrysanthemumˊChina Redˊ using the leaves of the aseptic seedings as explants. In order to establish an efficient genetic transformation system with leaves of ground-cover chrysanthemumˊChina Redˊ, several factors affected genetic transformation mediated by Agrobacterium were studied. The results showed that the highest rate of adventitious buds(89.6%) of ground-cover chrysanthemumˊChina Redˊwas obtained on MS + 2.0 mg•L-1 6-BA + 0.2 mg•L-1 NAA medium. The medium of 1/2MS+0.3 mg•L-1 NAA was the optimal for rooting, which had rooting rate of 100%. The conditions of genetic transformation: OD600=0.6, agrobacterium infection time for 7min, co- culture time for 48h, delayed screen time for 2 d, the kanamycin concentration was 15 mg•L-1 and the cefsulodin concentration was 300 mg•L-1. The establishment of high-efficiency transformation system laid a sound foundation for improving chrysanthemum by gene engineering.关键词
地被菊/叶片/再生体系/遗传转化体系Key words
ground-cover chrysanthemum/leaves/regeneration system/genetic transformation system分类
农业科技引用本文复制引用
毛洪玉,周杨,刘迪,贾红梅..地被菊‘中国红’再生及遗传转化体系的建立[J].沈阳农业大学学报,2015,(6):36-41,6.基金项目
辽宁省自然科学基金项目(2014027004) （2014027004）
