包装与食品机械Issue(6)：28-33,6.DOI:10.3969/j.issn.1005-1295.2015.06.007
玉米芯碱法提取木聚糖及其酶解制备低聚木糖的工艺研究
Studies on Alkali Extraction of Xylan from Corncobs and Preparation of Xylooligosaccharides by Xylanase Hydrolysis
何凤林 1沈生文 2曾颖 3刘欣4
作者信息
- 1. 广州泽力医药科技有限公司，广州 510000
- 2. 国家知识产权局专利局专利审查协作广东中心，广州 510000
- 3. 广州质量监督检测研究院，广州 510000
- 4. 华南农业大学，广州 510000
- 折叠
摘要
Abstract
The technological conditions of alkali extraction of xylan from corn cob were discussed in this paper by Studying the influence of alkali concentration,extracting temperature,extracting time,vibration speed and alcohol precipitation pH on the extraction rate.By means of single-factor experiment and orthobonal experi-ment ,the impacting factors that the content of reducing sugar,soluble total sugar and the average degree of pol-ymerization (DP)depended on the enzyme concentration ,pH value,substrate concentration,temperature and time have been studied.The results of experiments showed that:under the optimum conditions that enzyme con-centration of 0.06%(w/v),pH value of 4,substrate concentration of 12%(w/v),temperature of 40℃ and time of 8h,the content of soluble total sugar was 1 8.88mg/mL,the content of reducing sugar was 9.46 mg/mL and the average degree of polymerization (DP)was 1 .85.关键词
玉米芯/木聚糖/碱法/木聚糖酶/低聚木糖Key words
corncob/xylan/alkali methed/xylanase/xylooligosaccharide分类
轻工纺织引用本文复制引用
何凤林,沈生文,曾颖,刘欣..玉米芯碱法提取木聚糖及其酶解制备低聚木糖的工艺研究[J].包装与食品机械,2015,(6):28-33,6.
