摘要
Abstract
To establish a method for determination of the content of water - soluble polysaccharides in auxiliary hypoglycemic health food,the effects of extraction method,concentration of ethanol for precipi-tation,deproteinization method and coloration method on the determined values of polysaccharides content were studied.Result showed that the phenol -sulfuric acid method was chosen as the coloration method, with the conditions as follows:ultrasonic power 250 W,extraction time 60 min,80% ethanol concentra-tion for precipitation,and 1 /10 polysaccharide extraction volume,5% trichloroacetic acid concentration for deproteinization,deproteinization once,standing time 2 h,and the result was good.The standard curve regression equation was A =0.0144C -0.0655,the calibration curve had a good linearity in the range of 6.61 -79.28 μg/mL for glucose with the correlation coefficient (r)of 0.9996,and the average recovery at the spiked concentrations was 98.47% with RSD of 1.64%.The average content of water -soluble polysaccharides from the auxiliary hypoglycemic health food was 11.49% with RSD of 2.31%. This method was simple and accurate with a good repetion,which can be used to determine the content of polysaccharides in auxiliary hypoglycemic health products.关键词
水溶性多糖/测定方法/苯酚硫酸法/保健食品Key words
water -soluble polysaccharides/determination method/phenol - sulfuric acid method/health food分类
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