包装与食品机械2016，Vol.34Issue(1)：6-10,5.DOI:10.3969/j.issn.1005-1295.2016.01.002
马齿苋类黄酮提取工艺及抑菌效果的研究
Study on Extraction Process of Flavonoids from Purslane and Their Antibacterial Effect
摘要
Abstract
In order to optimize the ultrasonic assisted extraction process of flavonoids from purslane,the response surface methodology with three factors and three levels was used according to the principles of central composite design (Box-Benhnken).The optimum extraction process parameters were obtained by the software of design expert;The bacteriostatic effect of flavonoids from purslane against E.coli,Staphylococcus aureus and saccharomycetes were measured using filter-paper method,as follows:the ethanol volume fraction was 75%,the extracting time was 35min,and the tempreture was 35℃.Under these conditions,the value of flavone extraction yield was21.3517 mg/g approximating to the predicted value 21.8816 mg/g.The results indicated that the response surface methodology could be used to optimize the ultrasonic assisted extraction process of fla-vonoids from purslane;E.coli,Saccharomycetes were inhibited by the flavonoids from purslane,the MIC was 0.313mg/mL,and Staphylococcus aureus was strongly inhibited,the MIC was 0.156mg/mL.关键词
马齿苋/类黄酮/响应面法/提取工艺/抑菌效果Key words
purslane/flavonoids/response surface methodology/extraction process/antibacterial effect分类
轻工纺织引用本文复制引用
陈国妮,孙飞龙,闫亚茹..马齿苋类黄酮提取工艺及抑菌效果的研究[J].包装与食品机械,2016,34(1):6-10,5.基金项目
陕西省教育厅科研计划项目资助（15JK1315）；西安工程大学博士科研启动项目 （）
