茶叶科学2016，Vol.36Issue(2)：131-138,8.
SCoT标记分析陕西茶树资源的遗传多样性
Genetic Diversity Analysis of Tea Germplasm in Shaanxi Province Based on SCoT Marker
摘要
Abstract
Genetic diversity of 50 tea germplasm materials in Shaanxi was investigated. Taking 6 representative Shaanxi tea germplasm materials as templates, 38 of 121 SCoT marker primers were screened out, which showed specific bands and highly polymorphism. The results showed that SCoT markers could reveal high genetic diversity among tea germplasm, and each primer can detect out 4 to 17 alleles. On average, 11 allelic variations could be detected by each SCoT marker. 414 DNA fragments were amplified via 38 pairs of SCoT primer, of which 400 showed polymorphism and the ratio (PPB) was 96.62%. In addition, genetic polymorphism information content of each SCoT locus was between 0.56 and 0.99, the average of which was 0.90 for the tested materials, and the genetic similarity coefficient was between 0.59 and 0.71. The research showed that SCoT marker could be used in the genetic diversity research of tea germplasm. Basing on SCoT marker, genetic diversity level of these Shaanxi tea germplasms were relatively high in the genetic diversity.关键词
茶树/SCoT/多样性Key words
tea (Camellia sinensis)/SCoT/diversity分类
农业科技引用本文复制引用
陈熙,张羽,李佼,席彦军,张颜青..SCoT标记分析陕西茶树资源的遗传多样性[J].茶叶科学,2016,36(2):131-138,8.基金项目
陕西省自然科学基础研究计划项目(2013JZ008)、陕西省科技统筹创新工程(2013KTZB02-01-01)项目. （2013JZ008）
