茶叶科学2016，Vol.36Issue(2)：219-228,10.
茶树CsANS基因及其启动子的克隆与生物信息学分析
Cloning and Bioinformatical Analysis of Anthocyanin Synthase Gene and Its Promoter in Camellia sinensis
摘要
Abstract
The full length cDNA sequence of anthocyanin synthase gene(CsANS)was cloned from 'Wuyi qizhong C18' (Camellia sinensis) using rapid amplification of cDNA ends(RACE)technology. The promoter of CsANS was isolated using Genome Walking technology. The gene expression of ANS under different shading treatments was analyzed by the real-time PCR. The results showed that the full length cDNA of CsANS was 1000 bp, and it contained a 957 bp open reading frame (ORF), which encoding a protein of 320 amino acid, including two conservative functional domains, DIOX-N and 20G-Fell-Oxy. A sequence containing 1010 bp was isolated and found to be the promoter of CsANS, which contained many cis-acting elements related to anthocyanin biosynthetic pathway, such as the core promoter element TATA-box and light responsive element (including ACE, GT1-motif and Sp1), circadian (cis-acting regulatory element involved in circadian control). The real-time PCR analysis revealed that the gene was highly expressed under the sunshine (CK), while lowly expressed under the 75% shading. This study reveals that the expression of CsANS was regulated by light intensity.关键词
茶树/CsANS基因/启动子/生物信息学分析Key words
Camellia sinensis/CsANS gene/promoter/bioinformatics analysis分类
农业科技引用本文复制引用
金琦芳,陈志丹,孙威江,林馥茗,薛志慧,黄艳,唐秀华..茶树CsANS基因及其启动子的克隆与生物信息学分析[J].茶叶科学,2016,36(2):219-228,10.基金项目
国家"863"计划闽台优异茶树种质发掘创新与保护利用(2013AA10260605)、紫化芽叶茶树种质资源的挖掘保存与创新利用(2015N5008). （2013AA10260605）
