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发酵前及发酵后包被粪肠球菌微胶囊抗胁迫作用的研究

张琳 婷婷 綦文涛 李杰 李爱科

粮油食品科技2016,Vol.24Issue(2):80-84,5.
粮油食品科技2016,Vol.24Issue(2):80-84,5.

发酵前及发酵后包被粪肠球菌微胶囊抗胁迫作用的研究

Study on the stress resistance of encapsulated enterococcus faecalis pre -fermented and post -fermented

张琳 1婷婷 2綦文涛 1李杰 1李爱科3

作者信息

  • 1. 国家粮食局科学研究院,北京 100037
  • 2. 东北农业大学 动物科学技术学院,黑龙江 哈尔滨 150030
  • 3. 东北农业大学 动物科学技术学院,黑龙江 哈尔滨 150030
  • 折叠

摘要

Abstract

The enterococcus faecium were pre -fermented encapsulated by the method of emulsionand in-ternal gelation and post -fermented encapsulated by spraying dry.The stress resistance of the both micro-encapsulation formulations to the storage,high temperature,gastric juice and intestinal juice were evalua-ted compared with free enterococcus faecium.The results showed that the survival rates of pre -fermented encapsulated enterococcus faecium were 19.46% and 6.90% higher respectively than those of un -capsu-lated and post -fermented encapsulated enterococcus faecium after storage at room temperature for five months.The resistance of enterococcus faecium to the high temperature at 110 ℃ and 130 ℃ was signifi-cantly increased (P <0.05)by the pre -fermented encapsulation compared with post -fermented en-capsulation and free.The survival rates of pre -fermented encapsulated and post -fermented encapsula-ted enterococcus faecium were significantly increased (P <0.05)compared with those of uncoated ones when they were treated in the simulated gastric conditions for 30,90 and 180 min.The pre -fermented encapsulated was particularly evident.The results were similar in mimic environments of gastric juice and&nbsp;intestinal juice.The survival rates of pre -fermented encapsulated and post -fermented encapsulated en-terococcus faecium were increased 19.17% and 14.18%(P <0.05)compared with un -capsulated ones after they were treated in the simulated intestinal conditions for 180 min.These results suggest that the re-sistance of encapsulated enterococcus faecium is much higher than that of un -capsulated ones.The pre -fermented encapsulation can be a more effective way than post -fermented encapsulation for protecting probiotic microbes and has a high value of practical application.

关键词

微囊化益生菌/发酵前包被/发酵后包被/抗胁迫/粪肠球菌

Key words

microencapsulated probiotics/pre -fermented encapsulated/post -fermented encapsulated/stress resistance/enterococcus faecalis

分类

化学化工

引用本文复制引用

张琳,婷婷,綦文涛,李杰,李爱科..发酵前及发酵后包被粪肠球菌微胶囊抗胁迫作用的研究[J].粮油食品科技,2016,24(2):80-84,5.

基金项目

“十二五”农村领域国家科技计划课题(2013BAD10 B02);动物营养学国家重点实验室2013年度开放课题(2004DA125184F1306);北京市现代农业产业技术体系家禽创新团队资金 ()

粮油食品科技

OACSTPCD

1007-7561

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