食品科学技术学报2016，Vol.34Issue(2)：51-55,82,6.DOI:10.3969/j.issn.2095﹣6002.2016.02.008
体外酶法水解制备小分子透明质酸
Preparation of Small Molecular Hyaluronan by Enzymatic Hydrolysis in Vitro
原攀红 1康振 1金鹏 1刘龙 1堵国成 1陈坚2
作者信息
- 1. 江南大学 生物工程学院，江苏 无锡 214122
- 2. 江南大学 食品科学与技术国家重点实验室，江苏 无锡 214122
- 折叠
摘要
Abstract
In order to get concentrated distribution of small molecular hyaluronan, 6 g/L of high molecu﹣lar hyaluronic acid aqueous solution was prepared, added with a certain gradient of hyaluronidase, and controlling the hydrolysis time. The results showed that controlling the amount of hyaluronidase and hy﹣drolysis time, particular molecular weight hyaluronic acid could be prepared according to the law of the hyaluronan molecular weight decreased, which included tetrasaccharide and hexasaccharide. Further﹣more,through high performance size exclusion chromatography and agarose gel electrophoresis, we could prove that the molecular weight distribution of hydrolysate was concentrated.关键词
透明质酸/重组水蛭透明质酸酶/小分子透明质酸/高效体积排阻色谱/琼脂糖凝胶电泳Key words
hyaluronan/recombinant leech hyaluronidase/small molecular hyaluronan/high perform﹣ance size exclusion chromatography/agarose gel electrophoresis分类
轻工纺织引用本文复制引用
原攀红,康振,金鹏,刘龙,堵国成,陈坚..体外酶法水解制备小分子透明质酸[J].食品科学技术学报,2016,34(2):51-55,82,6.
