沈阳农业大学学报2016，Vol.47Issue(3)：347-351,5.DOI:10.3969/j.issn.1000-1700.2016.03.014
抗生素对松毛虫赤眼蜂产雌孤雌生殖品系的影响
Influence of Antibiotics on Thelytokous Strain of Trichogramma dendrolimi
摘要
Abstract
Laboratory tests were conducted by feeding Trichogramma dendrolimia adult thelytokous with different concentrations of tetracycline hydrochloride in honey to determine the removal effect of W olbachia, and explore the W olbachia influence on adult reproductive fitness. Thelytokous wasps were fed with three tetracycline concentrations (5, 15, 30 mg•mL-1) for five continuous generations, and spawning number, eclosion rate and female offspring percentage were measured. Chelex-100 was used to extract total DNA of the single wasp and Wolbachia molecular identification was conducted for detecting the completely removing of W olbachia from female adults with the surface of protein-coding genes wsp primers. The results showed that eclosion rate had a significant difference (p﹤0.05), overall trend of decrease then increase, and spawning number presented irregular change, moreover female offspring percentage showed decrease then increase trend among different generations. After antibiotic treatment with different concentrations, Wolbachia could be effectively removed with high concentration of tetracycline. With the same concentration of tetracycline treatment, the removal efficiency of wolbachia increased at first then decreased. This study suggested that Wolbachia can be removed by antibiotic, different concentrations of antibiotic treatment and processing time both affect W olbachia removal, high concentration have a remarkable removal efficiency. Antibiotic can break female parthenogenesis and affect eclosion rate.关键词
松毛虫赤眼蜂/Wolbachia/wsp基因/产卵量/羽化率/后代雌性百分比Key words
Trichrogramma dendrolimi/Wolbachia/wsp gene/spawning number/eclosinrate/female offspring percentage分类
生物科学引用本文复制引用
董辉,李帅强,谢丽娜,刘宁,丛斌..抗生素对松毛虫赤眼蜂产雌孤雌生殖品系的影响[J].沈阳农业大学学报,2016,47(3):347-351,5.基金项目
辽宁省自然科学基金项目(2015020768)；辽宁省教育厅一般项目(L2013267)；公益性行业科研专项项目(201303026)；国家科技支撑项目(2012BAD19B04) （2015020768）
