茶叶科学2016，Vol.36Issue(3)：268-276,9.
茯砖茶“金花”菌的分离、鉴定及转化葛根产物研究
Research on Separation, Identification and Transformation Products Transformed Kudzu Root of ′Jinhua′ Fungus
摘要
Abstract
In order to research whether the ′Jinhua′ fungus grow on and biotransformate kudzu root, get higher active fermentation products, one of advantages of strain was isolated from Fuzhuan Tea, named it as LS1. Pueraria lobata, its in the wild as the substrates in solid-state fermentation. DPPH radical scavenging capacities of fermentation products were investigated, making a determination of the contents of total flavone and fermentation products of puerarin were detected by high performance liquid chromatography. The result show that LS1 was identified preliminarily as Eurotium cristatum with GenBank accession number of KR812327 by morphological characteristics and the analysis of ITS-5.8S rDNA region. The contents of total flavone and puerarin were improved 15.97%、12.33%、20.69% and 20.48%、7%、20.29%, respectively.关键词
“金花”菌/葛根/发酵/鉴定/抗氧化/葛根素Key words
′Jinhua′ fungus/kudzu root/fermentation/identification/antioxidant activity/puerarin分类
轻工纺织引用本文复制引用
胡谢馨,易有金,柏连阳,李高阳,周红丽,周金伟,夏菠..茯砖茶“金花”菌的分离、鉴定及转化葛根产物研究[J].茶叶科学,2016,36(3):268-276,9.基金项目
国家科技支撑计划项目（2015BAD16B00、2015BAD16B01）、国家自然基金项目（31071738、31000827）、湖南省教育厅重点项目（15A082）。 （）
