茶叶科学2016，Vol.36Issue(3)：312-322,11.
茶树两个 Dof 转录因子的分离及其在温度胁迫中的响应分析
Isolation and Expression Analysis of Two Temperature Responsive Dof Genes from Camellia sinensis
摘要
Abstract
Tea is an important economic crop. Tea production is affected by temperature factors. CsDof1 and CsDof2, which encode Dof transcription factors, were cloned from tea cultivar ‘Yingshuang’ [Camellia sinensis (L.) O. Kuntze]. The nucleotide and amino acid sequences, phylogenetic tree and molecular models of the CsDof1 and CsDof2 were analyzed. The results showed that the CsDof1 and CsDof2 are 1389 and 1458 nucleotides in length, which encode 463 and 486 amino acids respectively. Typical zinc finger domains are present in both CsDof1 and CsDof2, from coden 133 to 195 and 124 to 186 respectively. The CsDof1 and CsDof2 were hydrophilic proteins. The three-dimensional structures of CsDof1 and CsDof2 indicated the presence of a highly conserved Dof domain with four cysteine residues. Quantitative real-time PCR analysis showed that CsDof1 and CsDof2 were induced by different temperature stresses and the expression profiles were different among tea cultivars.关键词
茶树/Dof 转录因子/锌指结构/温度胁迫/基因表达Key words
Camellia sinensis/Dof transcription factor/zinc finger/temperature stress/gene expression分类
农业科技引用本文复制引用
李辉,黄蔚,刘志薇,王永鑫,吴致君,庄静..茶树两个 Dof 转录因子的分离及其在温度胁迫中的响应分析[J].茶叶科学,2016,36(3):312-322,11.基金项目
国家自然科学基金 （）
