茶叶科学2016，Vol.36Issue(3)：323-329,7.
茶假眼小绿叶蝉抗联苯菊酯品系和敏感品系解毒酶活性及增效作用研究
Activities of the Detoxifying Enzymes and Synergism in the Bifenthrin-resistant and Susceptible Strains of Tea Leafhopper, Empoasca vitis
摘要
Abstract
Tea green leafhopper, Empoasca vitis, is the most serious pest in tea gardens. The detoxifying enzyme activities and synergism in the bifenthrin-resistant and susceptible leafhopper were measured via biochemical methods and bioassays to clarify their potential relationship to the resistance of tea leafhopper to bifenthrin. The detoxifying enzyme activity tests showed that the activities of carboxylesterase (CarE), Cytochrome P450-mediated O-demethylation activity toward p-nitroanisole (PNOD) and glutathione-S-transferase (GST) in the resistant strain were all higher than those in susceptible strain. The activit ies of the three detoxifying enzymes in bifenthrin-resistant strain were 2.09, 4.34 and 1.59 folds those of the susceptible strain respectively. Synergism tests showed that the synergist ratios of triphenyl phosphate (TPP), piperonyl buoxide (PBO) and diethyl maleate (DEM) were 1.04, 1.09, and 1.00 folds respectively in the bifenthrin-susceptible strain and 1.80, 7.97 and 1.03 foldsrespectively in the bifenthrin-resistant strain. These results indicate that CarE and PNOD play important roles in bifenthrin resistance of tea leafhoppers.关键词
茶假眼小绿叶蝉/联苯菊酯/抗性/解毒酶/增效作用Key words
Empoasca vitis/bifenthrin/resistance/detoxifying enzymes/synergism分类
农业科技引用本文复制引用
李建宇,史梦竹,傅建炜,王婷,张志康..茶假眼小绿叶蝉抗联苯菊酯品系和敏感品系解毒酶活性及增效作用研究[J].茶叶科学,2016,36(3):323-329,7.基金项目
福建省自然科学基金 （）
